Cullin 5 (CUL5) (N-Term), (AA 4-18) antibody

Details for Product No. ABIN237097
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Antigen
Synonyms
cul5, wu:fd17d09, wu:fi20h12, xx:11fd17d09, zgc:66185, Xcullin5, CG1401, CUL5, Cul5, Cullin 5, Cullin5, Dmel\\CG1401, cul-5, vacm-1, vacm1, VACM-1, VACM1, 4921514I20Rik, 8430423K24Rik, AI852817, C0300 ... show more
cul5, wu:fd17d09, wu:fi20h12, xx:11fd17d09, zgc:66185, Xcullin5, CG1401, CUL5, Cul5, Cullin 5, Cullin5, Dmel\\CG1401, cul-5, vacm-1, vacm1, VACM-1, VACM1, 4921514I20Rik, 8430423K24Rik, AI852817, C030032G03Rik, C330021I08Rik, Cullin-5 show less
Epitope
N-Term, AA 4-18
(8), (6), (3), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(49), (32), (29), (25), (23), (11), (2), (1), (1), (1)
Host
Rabbit
(48), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(26), (19), (14), (12), (7), (7), (3), (3), (2)
Pubmed 5 references available
Quantity 100 µL
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Catalog No. ABIN237097
669.53 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

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Immunogen A synthetic peptide corresponding to AA 4-18 of Human Cul5 (N-terminus) coupled to KLH. Sequence: SNLLKNKGSLQFEDK
Specificity Reacts with human Cullin 5. Cross reactivity with other human cullins may occur.
Characteristics Rabbit anti Cul5 (AA 4-18), Rabbit antibody to Cullin 5 (Cul5), N-terminal (AA 4-18)
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using 293T, U2OS and others). This antibody also co-immunoprecipitates associated proteins. A 90.9 kDa band corresponding to human Cul5 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Concentration Total Protein: 85 mg/mL (Refractometry)
Buffer 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Aliquot and store at -20°C or below.
General Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Querido, Blanchette, Yan et al.: "Degradation of p53 by adenovirus E4orf6 and E1B55K proteins occurs via a novel mechanism involving a Cullin-containing complex." in: Genes & development, Vol. 15, Issue 23, pp. 3104-17, 2001 (PubMed).

Yu, Yu, Liu et al.: "Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex." in: Science (New York, N.Y.), Vol. 302, Issue 5647, pp. 1056-60, 2003 (PubMed).

Le Meignen, Mossler, Mates et al.: "[Management of immune thrombocytopenic purpura: A prospective study of 147 children from the regional network RH" in: Archives de pediatrie : organe officiel de la Societe francaise de pediatrie, 2008 (PubMed).

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