anti-Cullin 7 antibody (CUL7) (C-Term)

Details for Product anti-CUL7 Antibody No. ABIN237098
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C-Term, AA 1679-1698
(14), (6), (4), (2), (2), (1), (1), (1), (1), (1)
(46), (18), (17)
(42), (4)
This Cullin 7 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(31), (21), (11), (10), (10), (8), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μL
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Immunogen A synthetic peptide corresponding to AA 1679-1698 of Human Cul7 (C-terminus) coupled to KLH. Sequence: RSRGVPYASCTATQSFSTFR
Specificity Reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur.
Characteristics Rabbit anti Cul7 (AA 1679-1698), Rabbit antibody to Cullin 7 (Cul7), C-terminal (AA 1679-1698)
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3 and others). This antibody also co-immunoprecipitates associated proteins in overexpressed CUL7 systems. A 191.2 kDa band corresponding to human Cul7 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Upon receipt, aliquot and store at -20 °C or below.
General Dias, Dolios, Wang et al.: "CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16601-6, 2002 (PubMed).

Catalog No. ABIN237098
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