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Details for Product No. ABIN237099

Cullin Associated and Neddylation Dissociation Protein 1 (CAND1) (N-Term), (AA 11-24) antibody

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Antigen
Epitope
N-Term, AA 11-24
Reactivity
Human, Rat (Rattus), Mouse (Murine)
Host
Rabbit
Clonality Polyclonal
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN237099
Quantity 100 µL
Price
669.53 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Availability Will be delivered in 4 to 5 Business Days
Immunogen A synthetic peptide corresponding to AA 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH. Sequence: LLEKMTSSDKDFRF
Specificity Reacts with human, rat and mouse CAND1/TIP120A. It also cross-reacts with human, rat and mouse CAND2/TIP120B.
Characteristics Rabbit anti CAND1, N-terminal, Rabbit antibody to Human Cullin Associated and Neddylation Dissociation Protein 1 (CAND1), N-terminal
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered

Application Details

Handling References back to top
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000), Western blot (1:500 - 1:1.000) and Immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa and NIH-3T3 and others). A 136.4 kDa band corresponding to human CAND1 is detected. Most cell lines expressing CAND1 can be used as a positive control. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Concentration Total Protein: 85 mg/mL (Refractometry)
Buffer 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Prepare working dilution only prior to immediate use. Avoid multiple freeze/thaw cycles
Storage 4 °C/-20 °C
Storage Comment This product is stable for several weeks as an undiluted liquid when stored at 2-8°C. Aliquot and store at -20°C.
Makino, Yoshida, Yogosawa et al.: "Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA-binding protein and a novel transcriptional activator, TIP120." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 4, Issue 9, pp. 529-39, 1999 (PubMed).

Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Zheng, Yang, Harrell et al.: "CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex." in: Molecular cell, Vol. 10, Issue 6, pp. 1519-26, 2002 (PubMed).

Oshikawa, Matsumoto, Yada et al.: "Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1." in: Biochemical and biophysical research communications, Vol. 303, Issue 4, pp. 1209-16, 2003 (PubMed).

Feng, Shen, Sullivan et al.: "Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation." in: The Plant cell, Vol. 16, Issue 7, pp. 1870-82, 2004 (PubMed).

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