Cullin-Associated and Neddylation-Dissociated 2 (Putative) (CAND2) (C-Term), (AA 1130-1143) antibody

Details for Product No. ABIN237100
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Synonyms cand1, si:ch211-257f16.2, si:dkey-72g22.1, CAND2, TIP120B, Tp120b, 2210404G23Rik, mKIAA0667, Tip120B
C-Term, AA 1130-1143
(5), (3), (1), (1)
Human, Rat (Rattus), Mouse (Murine)
(10), (4), (4), (2), (2), (2), (2), (2), (1), (1)
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(10), (6), (5), (3)
Pubmed 5 references available
Quantity 100 µL
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Catalog No. ABIN237100
669.53 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

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Immunogen A synthetic peptide corresponding to AA 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH. Sequence: DSASAPSTDSMELS
Specificity Reacts with human, rat and mouse CAND2/TIP120B. Cross reacts with human, rat and mouse CAND1/TIP120A. Cross reactivity with CAND2 from other sources is not known.
Characteristics Rabbit anti CAND2, C-terminal, Rabbit antibody to Human Cullin Associated and Neddylation Dissociated Protein 2 (CAND2), C-terminal
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Alternative Name Cullin Associated and Neddylation Dissociated Protein 2 (CAND2)
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from transfected cell lysates (using HeLa and NIH-3T3 and others). Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa band corresponding to human CAND2 is detected. CAND2 is specifically expressed in muscle and heart tissue. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Concentration Total Protein: 85 mg/mL (Refractometry)
Buffer 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Aliquot and store at -20°C.
General Makino, Yoshida, Yogosawa et al.: "Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA-binding protein and a novel transcriptional activator, TIP120." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 4, Issue 9, pp. 529-39, 1999 (PubMed).

Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Zheng, Yang, Harrell et al.: "CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex." in: Molecular cell, Vol. 10, Issue 6, pp. 1519-26, 2002 (PubMed).

Oshikawa, Matsumoto, Yada et al.: "Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1." in: Biochemical and biophysical research communications, Vol. 303, Issue 4, pp. 1209-16, 2003 (PubMed).

Feng, Shen, Sullivan et al.: "Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation." in: The Plant cell, Vol. 16, Issue 7, pp. 1870-82, 2004 (PubMed).

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