Escherichia Coli (O and K Antigenic Serotypes) (E. coli) antibody (FITC)

Antigen: Escherichia Coli (O and K Antigenic Serotypes)

Epitope: E. coli

Clonality: Polyclonal

Host: Goat

Conjugate: FITC

Application: ELISA, Immunofluorescence (IF)

Catalog no.: ABIN237175

Additional Information

Alternative name: Escherichia coli (E. coli), O and K antigenic serotypes

Immunogen: Mixture of E. coli serotypes

Format: Liquid

Description: Goat anti E. coli (all antigens) Goat Antibody to Escherichia coli (E. coli), O and K antigenic serotypes Fluorescein conjugated

Specificity: Many O and K antigenic serotypes of Escherichia coli. Antiserum is not absorbed and may react with related Enterobacteriaceae

Application Details

Application Notes: Suitable for use in ELISA and direct IFA. Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Catalog K65701R and K65702R). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 4?5mg/ml (OD280nm, E0.1% 1.4)

Purification: IgG fraction covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Buffer: 0.01M PBS, pH 7.2 containing 10mg/ml BSA. Preservative: 0.1% Sodium azide

Storage: Short-term (up to 6 months) store at 2-8°C under subdued light. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only