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Application Notes
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ELISA: To detect human HCC-1 by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant human HCC-1. Western blot: To detect human HCC-1 by Western blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human HCC-1 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Reconstitution: Reconstitute in sterile water to a concentration of >0.2mg/ml.
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Concentration
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1mg/ml (prior to lyophilization)
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Purification
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Affinity chromatography using immobilized human HCC-1 matrix. Product is sterile filtered.
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Buffer
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Lyophilized from 0.5X PBS, pH 7.4 Preservative: None
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Restrictions
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For Research Use only
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