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p53-Associated, Parkin-Likeprotein (PARC) (AA 2503-2517), (C-Term), (Cytoplasmic) antibody

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C-Term, AA 2503-2517, Cytoplasmic
Human, Mouse (Murine)
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 1 reference available
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Quantity 100 μL
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Immunogen A synthetic peptide corresponding to AA 2503-2517 of Human PARC (C-terminus) coupled to KLH. Sequence: FFFGDEEEDEDEAYD
Specificity Reacts with human and mouse PARC. Cross reactivity with PARC from other sources is not known.
Characteristics Rabbit anti PARC (AA 2503-2517), Rabbit antibody to Human p53-associated, Parkin-like Cytoplasmic protein (PARC) (amino acid 2503-2517)
Purification Neat antiserum delipidation and defibrination.
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000), Western blot (1:500 - 1:1.000) and Immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3 and others). Coimmunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or below.
General Nikolaev, Li, Puskas et al.: "Parc: a cytoplasmic anchor for p53." in: Cell, Vol. 112, Issue 1, pp. 29-40, 2003 (PubMed).

Catalog No. ABIN237917

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