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Details for Product No. ABIN237917

p53-Associated, Parkin-Likeprotein (PARC) (C-Term), (AA 2503-2517), (Cytoplasmic) antibody

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Antigen
Epitope
C-Term, AA 2503-2517, Cytoplasmic
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality Polyclonal
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN237917
Quantity 100 µL
Price
594.73 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Availability Will be delivered in 4 to 5 Business Days
Immunogen A synthetic peptide corresponding to AA 2503-2517 of Human PARC (C-terminus) coupled to KLH. Sequence: FFFGDEEEDEDEAYD
Specificity Reacts with human and mouse PARC. Cross reactivity with PARC from other sources is not known.
Characteristics Rabbit anti PARC (AA 2503-2517), Rabbit antibody to Human p53-associated, Parkin-like Cytoplasmic protein (PARC) (amino acid 2503-2517)
Purification Neat antiserum delipidation and defibrination.

Application Details

Handling References back to top
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000), Western blot (1:500 - 1:1.000) and Immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3 and others). Coimmunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Aliquot and store at -20°C or below.
Nikolaev, Li, Puskas et al.: "Parc: a cytoplasmic anchor for p53." in: Cell, Vol. 112, Issue 1, pp. 29-40, 2003 (PubMed).

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