p53-associated, Parkin-likeprotein (PARC) (AA 2503-2517,Cytoplasmic) antibody

Antigen: p53-associated, Parkin-likeprotein (PARC)

Binding Site: AA 2503-2517,Cytoplasmic

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN237917

Additional Information

Immunogen: A synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH. Sequence: FFFGDEEEDEDEAYD

Format: Liquid

Description: Rabbit anti PARC (a.a. 2503-2517) Rabbit Antibody to Human p53-associated, Parkin-like Cytoplasmic protein (PARC) (amino acid 2503-2517)

Specificity: Reacts with human and mouse PARC. Cross reactivity with PARC from other sources is not known.

Application Details

Application Notes: Suitable for use in ELISA (1:2,000 ? 1:10,000), Western blot (1:500 ? 1:1,000) and Immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3 and others). Coimmunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Total Protein: Lot specific (Refractometry)

Purification: Neat antiserum delipidation and defibrination.

Buffer: Not applicable. Preservative: 0.01% (w/v) Sodium Azide

Storage: Aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use. : This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Restrictions: For Research Use only

Publications

Nikolaev, Li, Puskas et al.: "Parc: a cytoplasmic anchor for p53." in: Cell, Vol. 112, Issue 1, pp. 29-40, 2003 (PubMed).