Pepsin antibody (HRP)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Conjugate
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Application
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Western Blotting (WB) (31), ELISA (21), Immunoprecipitation (IP) (14), Enzyme Immunoassay (EIA) (10), Immunofluorescence (IF) (8), Immunohistochemistry (IHC) (7), Dot Blot (Dot) (3), Immunodiffusion (ID) (3), Radioimmunoassay (RIA) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), ELISA (Detection) (1), Immunoelectrophoresis (IEP) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN237939 |
| Quantity |
20mg (10mg/ml (OD280nm) (prior to lyophil...) |
| Price |
596.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Pepsin (Pig Stomach)
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Immunogen
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Pepsin (Pig Stomach)
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Format
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Lyophilized
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Description
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Goat A’ Pepsin (Pig Stomach) Goat Antibody to Pepsin (Pig Stomach) Horseradish Peroxidase conjugated
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Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin (Porcine Stomach). Cross-reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.
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Application Notes
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Assayed against 1.0ug of Pepsin (Porcine Stomach) in a standard ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Concentration
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10mg/ml (OD280nm) (prior to lyophilization)
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Purification
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Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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Buffer
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Lyophilized from 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 containing 10mg/ml BSA (IgG and Protease free). Preservative: 0.01% (w/v) Gentamicin Sulfate
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Storage
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Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks at 2-8°C as an undiluted liquid. Prepare working dilution only prior to immediate use. For extended storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles. Expiration date is one (1) year from reconstitution date. Reference: The references listed below are for research purposes only. Stovold, R., et al., (2007), ?Pepsin, a Biomarker of Gastric Aspiration in Lung Allografts?, Am. J. Respir. Crit. Care Med.?, 175: 1298-1303 Tasker, A., et al., (2002), ?Is Gastric Reflux a Cause of Otitis Media With Effusion in Children??, Laryngoscope, 112: 1930-1934 Farr & Nakane, (1981), J. Immunol. Methods, 47: 129-144.Reconstitute with 2ml deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature.
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Restrictions
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For Research Use only
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Alternatives for antigen "Pepsin", type "Antibodies"
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Hosts
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Goat (23), Rabbit (8), Mouse (2)
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Reactivities
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Pig (Porcine) (19), Human (4)
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Applications
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Western Blotting (WB) (31), ELISA (21), Immunoprecipitation (IP) (14), Enzyme Immunoassay (EIA) (10), Immunofluorescence (IF) (8), Immunohistochemistry (IHC) (7), Dot Blot (Dot) (3), Immunodiffusion (ID) (3), Radioimmunoassay (RIA) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), ELISA (Detection) (1), Immunoelectrophoresis (IEP) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (8), HRP (4)
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Alternatives HRP 
