Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1) (C-Term), (AA 94-108) antibody

Details for Product No. ABIN238044
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Antigen
Epitope
C-Term, AA 94-108
(18), (2), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(52), (24), (17), (14), (2), (1), (1)
Host
Rabbit
(50), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(38), (18), (13), (10), (8), (7), (5), (2), (2), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN238044
702.53 $
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Immunogen A synthetic peptide corresponding to AA 94-108 of Human ROC1 (C-terminal) coupled to KLH. Sequence: CPLDNREWEFQKYGH
Specificity Reacts with human, mouse, C. elgans and zebra fish ROC1.
Characteristics Rabbit anti ROC1 (AA 94-108), Rabbit antibody to Human Regulator of Cullins 1 (ROC1) (AA 94-108)
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Alternative Name Regulator of Cullins 1 (ROC1)
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3 and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation of ubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Concentration Total Protein: 85 mg/mL (Refractometry)
Buffer 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or below.
General Kamura, Koepp, Conrad et al.: "Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase." in: Science (New York, N.Y.), Vol. 284, Issue 5414, pp. 657-61, 1999 (PubMed).

Furukawa, Zhang, McCarville et al.: "The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1." in: Molecular and cellular biology, Vol. 20, Issue 21, pp. 8185-97, 2000 (PubMed).

Jung, Park, Pae et al.: "[Short-term therapy with pegylated interferon plus ribavirin for the chronic hepatitis C genotype 2 patients]" in: The Korean journal of hepatology, Vol. 13, Issue 3, pp. 341-8, 2007 (PubMed).

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