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Details for Product No. ABIN238044

Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1) (C-Term), (AA 94-108) antibody

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Antigen
Synonyms BA554C12.1, RNF75, ROC1, hop21
Epitope
»Alternatives C-Term, AA 94-108
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN238044
Quantity 100 µL
Price
669.53 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Availability Will be delivered in 4 to 5 Business Days
Immunogen A synthetic peptide corresponding to AA 94-108 of Human ROC1 (C-terminal) coupled to KLH. Sequence: CPLDNREWEFQKYGH
Specificity Reacts with human, mouse, C. elgans and zebra fish ROC1.
Characteristics Rabbit anti ROC1 (AA 94-108), Rabbit antibody to Human Regulator of Cullins 1 (ROC1) (AA 94-108)
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Alternative Name Regulator of Cullins 1 (ROC1)
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3 and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation of ubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Concentration Total Protein: 85 mg/mL (Refractometry)
Buffer 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Aliquot and store at -20°C or below.
Kamura, Koepp, Conrad et al.: "Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase." in: Science (New York, N.Y.), Vol. 284, Issue 5414, pp. 657-61, 1999 (PubMed).

Furukawa, Zhang, McCarville et al.: "The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1." in: Molecular and cellular biology, Vol. 20, Issue 21, pp. 8185-97, 2000 (PubMed).

Jung, Park, Pae et al.: "[Short-term therapy with pegylated interferon plus ribavirin for the chronic hepatitis C genotype 2 patients]" in: The Korean journal of hepatology, Vol. 13, Issue 3, pp. 341-8, 2007 (PubMed).

Alternatives for antigen "Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1)", type "Antibodies"
Hosts (47), (1)
Reactivities (46), (24), (15), (14), (1), (1), (1)
Applications (32), (20), (13), (11), (10), (5), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (14), (2), (1), (1), (1), (1), (1)
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