S-Phase Kinase-Associated Protein 1 (SKP1) (amino acids 152-163) antibody

Antigen: S-Phase Kinase-Associated Protein 1 (SKP1)

Synonyms: OCP2, p19A, EMC19, SKP1A, OCP-II, TCEB1L, MGC34403, ASK18, F14N23.11, F14N23_11, SK18, SKP1-like 18, ASK4, SK4, SKP1-like 4, T20H2.8, T20H2_8, SKP1, 15kDa, Tceb1l, p19Skp1, 2610043E24Rik, 2610206H23Rik, Skp1a, skp1a, MGC64317, MGC73186, zgc:73186, ocp2, emc19, ocp-ii, tceb1l, MGC127638, ARABIDOPSIS SKP1 HOMOLOGUE 1, ASK1, ATSKP1, S PHASE KINASE-ASSOCIATED PROTEIN 1, T4O12.17, T4O12_17, UFO INTERACTING PROTEIN 1, UIP1, ocp-2, DKFZp459G0415

Epitope: amino acids 152-163

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN238117

Additional Information

Alternative name: S-phase Kinase-associated Protein 1A (SKP1) (amino acids 152-163)

Immunogen: A synthetic peptide corresponding to amino acids 152-163 of human SKP1 (C-terminus) coupled to KLH. Sequence: QVRKENQWCEEK

Format: Neat antiserum, Liquid

Description: Rabbit anti SKP1 (a.a. 152-163) Rabbit Antibody to S-phase Kinase-associated Protein 1A (SKP1) (amino acids 152-163)

Specificity: Reacts with Human SKP1. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.

Synonyms: OCP2, p19A, EMC19, SKP1A, OCP-II, TCEB1L, MGC34403, ASK18, F14N23.11, F14N23_11, SK18, SKP1-like 18, ASK4, SK4, SKP1-like 4, T20H2.8, T20H2_8, SKP1, 15kDa, Tceb1l, p19Skp1, 2610043E24Rik, 2610206H23Rik, Skp1a, skp1a, MGC64317, MGC73186, zgc:73186, ocp2, emc19, ocp-ii, tceb1l, MGC127638, ARABIDOPSIS SKP1 HOMOLOGUE 1, ASK1, ATSKP1, S PHASE KINASE-ASSOCIATED PROTEIN 1, T4O12.17, T4O12_17, UFO INTERACTING PROTEIN 1, UIP1, ocp-2, DKFZp459G0415

Application Details

Application Notes: Suitable for use in ELISA (1:2,000 ? 1:10,000) and Western blot (1:500 ? 1:1,000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3 and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band corresponding to human SKP1 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Total Protein: 85mg/ml (Refractometry)

Purification: Delipidation and defibrination Product is sterile filtered.

Buffer: Not applicable. Preservative: 0.01% (w/v) Sodium Azide

Storage: Upon receipt, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use. : This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Restrictions: For Research Use only

Publications

Publications: Zhang, Kobayashi, Galaktionov et al.: "p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase." in: Cell, Vol. 82, Issue 6, pp. 915-25, 1995 (PubMed).

Connelly, Hieter: "Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression." in: Cell, Vol. 86, Issue 2, pp. 275-85, 1996 (PubMed).

Feldman, Correll, Kaplan et al.: "A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p." in: Cell, Vol. 91, Issue 2, pp. 221-30, 1997 (PubMed).

Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Le Meignen, Mossler, Mates et al.: "[Management of immune thrombocytopenic purpura: A prospective study of 147 children from the regional network RH" in: Archives de pediatrie : organe officiel de la Societe francaise de pediatrie, 2008 (PubMed).