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S-Phase Kinase-Associated Protein 1A (SKP1A) (AA 152-163), (C-Term) antibody

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C-Term, AA 152-163
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(1), (1)
Pubmed 5 references available
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Quantity 100 μL
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Immunogen A synthetic peptide corresponding to AA 152-163 of human SKP1 (C-terminus) coupled to KLH. Sequence: QVRKENQWCEEK
Specificity Reacts with Human SKP1. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.
Characteristics Rabbit anti SKP1 (AA 152-163), Rabbit antibody to S-phase Kinase-associated Protein 1A (SKP1) (AA 152-163)
Purification Neat antiserum delipidation and defibrination.
Sterility Sterile filtered
Alternative Name S-phase Kinase-associated Protein 1A (SKP1)
Application Notes Suitable for use in ELISA (1:2.000 - 1:10.000) and Western blot (1:500 - 1:1.000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3 and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band corresponding to human SKP1 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Buffer 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage -20 °C
Storage Comment Upon receipt, aliquot and store at -20 °C or below.
General Le Meignen, Mossler, Mates et al.: "[Management of immune thrombocytopenic purpura: A prospective study of 147 children from the regional network RH" in: Archives de pediatrie : organe officiel de la Societe francaise de pediatrie, 2008 (PubMed).

Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Feldman, Correll, Kaplan et al.: "A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p." in: Cell, Vol. 91, Issue 2, pp. 221-30, 1997 (PubMed).

Connelly, Hieter: "Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression." in: Cell, Vol. 86, Issue 2, pp. 275-85, 1996 (PubMed).

Zhang, Kobayashi, Galaktionov et al.: "p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase." in: Cell, Vol. 82, Issue 6, pp. 915-25, 1995 (PubMed).

Catalog No. ABIN238117

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