Progesterone Receptor (PGR) (pSer190) antibody

Antigen: Progesterone Receptor (PGR)

Synonyms: PR, NR3C3, PR-A, PR-B, BB114106, 9930019P03Rik, ENSMUSG00000074510, PGR, pgr-A, XPR-1, LOC443444, pg, pr, gb:dq017620

Epitope: pSer190

Clonality: Monoclonal (1154)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN238337

Additional Information

Alternative name: Progesterone receptor

Swiss-Prot: P06401

Immunogen: Synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer190 of human progesterone receptor

Cross-Reactivity: Human

Clone: 1154

Description: There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.

Specificity: This antibody is specific for Progesterone Receptor phospho Ser190.

Application Details

Application Notes: IHC (1:1000-1:2000). Dot blots (1:1000 to 1:2000) and Western blots (1:1000 to 1:2000). Recommended Positive Control: T47D treated with sythetic progestin agonist R5020 (500 nM). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Aff - Purified

Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol

Storage: For long-term storage -80°C is recommended, but short term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Hormones, Receptors, Phospho-specific antibodies

Restrictions: For Research Use only