Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2) (pSer218,pSer222) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2)

Epitope: pSer218,pSer222

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN238350

Additional Information

Characteristics: Synonyms: MEK1, MEK2, MKK2, MKK1, PRKMK1, PRKMK2

Alternative name: MAPKK1 / MAPKK 2

Immunogen: Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of humanMAPK Kinase 1. Remarks: The immunogen sequence is highly conserved within species (human, mouse,rat, hamster, bovine etc. ).

Cross-Reactivity: Human

Description: MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of threecomponents of MAP kinase cascade - an important signaling pathway that regulates cellgrowth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of twoserine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificitykinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK,mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical stepin the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus,where it regulates gene expression by activating transcription factors.

Specificity: The polyclonal antibody reacts with MEK 1/2. MEK 1 and MEK 2 are integral components ofthe MAP kinase cascade, an important pathway for cell growth and differentiation. Species: Human. Others not tested.

Application Details

Application Notes: Western Blotting under reducing and non-reducing conditions: Recommended dilution: 0.5 μg/mlPositive material: RAJI human Burkitt lymphoma cell lineSample preparation: Resuspend approx.50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing/reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. Reducing conditions are recommended. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v)high-grade BSA (Protease free) as stabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch

Restrictions: For Research Use only