Tumor Necrosis Factor (TNF) antibody (Biotin)

Antigen: Tumor Necrosis Factor (TNF)

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN238351

Additional Information

Characteristics: Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

Alternative name: Tumor necrosis factor (TNF-alpha)

Swiss-Prot: P01375

Immunogen: Highly pure (>98%) recombinant hTNF-α

Cross-Reactivity: Human

Specificity: Species: Human. Others not tested. Add. Information: ElisaDirect: To detect hTNF-alpha by direct ELISA (using 100 μl/well antibody solution) aconcentration of 0. 25 - 1. 0 μg/ml ofhis antibody is required. This biotinylated polyclonal antibody, in conjunction withcompatible secondary reagents, allows the detection of at least 0. 2 - 0. 4 ng/well ofrecombinant hTNF-alpha. Sandwich: To detect hTNF-alpha by sandwich ELISA (using 100 μl/well antibody solution) aconcentration of 0. 25 - 1. 0 μg/ml of this antibody is required. This biotinylated polyclonalantibody, in conjunction with PeproTech's Polyclonal Anti-Human TNF-alpha (500-P31AG)as a capture antibody, allows the detection of at least 0. 2 - 0. 4 ng/well of recombinanthTNF-alpha. Western Blot0. 1 - 0. 2 µg/ml. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTNF-α is 1. 5 - 3. 0 ng/lane, under either reducing or non-reducingconditions.

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Application Details

Application Notes: ElISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS, pH 7.2Label: BiotinReconstitution: Reconstitute in sterile PBS containing 0.1% BSA to a concentration of0.1-1.0 mg/ml.

Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. Thereconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stablefor at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Research Area: Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology

Restrictions: For Research Use only