phospholipid Scramblase 1 (PLSCR1) antibody

Antigen: phospholipid Scramblase 1 (PLSCR1)

Synonyms: MMTRA1B, Nor1, Tra1, Tra1a, Tra1b, Tras1, Tras2, MmTRA1a, MmTRA1b, MGC93252, PLSCR1, PLSCR2, MGC128844, mmtra1b, MGC84969, MGC148322

Clonality: Monoclonal (13A6 [TEC-23])

Host: Mouse

Reactivity: Rat (Rattus)

Catalog no.: ABIN238363

Additional Information

Characteristics: Synonyms: Phospholipid Scramblase 1

Alternative name: PLSCR1

Immunogen: BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells. Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cellsof immunized mice using standard procedures.

Cross-Reactivity: Rat (Rattus)

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 13A6 [TEC-23]

Description: PLSCR1 (Phospholipid Scramblase 1) is a multiply palmitoylated endofacial plasmamembrane protein containing several SH3 and WW domain binding motives. In the plasmamembrane, PLSCR1 plays a role in transbilayer lipid redistributions and signaltransduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bindDNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highlyinduced by interferons and growth factors.

Specificity: This antibody binds to rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDamembrane-associated protein accelerating bidirectional movement of plasma membranephospholipids during conditions of elevated calcium. Species: Rat. Others not tested.

Application Details

Protocol: Western Blot: Positive control: RBL rat basophilic leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% TritonX-100 in 20 mM Tris/Cl, 100 mM NaCl, 2 mM EDTA, 2 mM Na3Vo4, 10 mMglycerolphosphate, pH 8. 0, 1 mM PMSF, 10 μg/ml aprotinin). Incubate 20 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing LaemmliSDS-PAGE sample buffer. Boil for 5 min. Note: Both reducing and non-reducing conditions

Application Notes: Immunoprecipitation. Western Blotting: 1 μg/ml. Flow Cytometry. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Ascites

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only