HLA Class I alpha G (HLAG) antibody

Antigen: HLA Class I alpha G (HLAG)

Clonality: Monoclonal (87G)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Functional Studies (Func)

Catalog no.: ABIN238376

Additional Information

Characteristics: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Alternative name: HLA class I alpha G / HLA-G

Swiss-Prot: P17693

Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected withand expressing genes encoding HLA-G and human beta2-microglobulin.

Cross-Reactivity: Human

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 87G

Description: HLA-G belongs to the HLA class I heavy chain paralogues (MHC Class Ib, nonclassical). It isa heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta2 microglobulin). Alternate splicing provides both soluble and membrane bound forms ofHLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be partof the fetal protection system and is expressed on fetal derived placental cells. It is expressed on the surface of trophoblast cells.

Specificity: ABIN238376 recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 andHLA-G5). This antibody blocks interaction of HLA-G with inhibitory receptors. Species Reactivity: Tested: Human. Does not reacts with Mouse and Rat.

Application Details

Application Notes: Flow Cytometry. Positive control: JEG-3 human choriocarcinoma epithelial cell lineImmunohistochemistry (Frozen Sections): 10 µg/ml (20 min at 25°C). Positive tissue: extravillous cytotrophoblastELISA: Has been tested as the Capture antibody in a sandwich ELISA for analysis of humanHLA-G in combination with antibody W6/32. Recommended Dilution: 1 µg/mlPositive Control: JEG-3 human choriocarcinoma epithelial cell line. Functional Application: This antibody 87G blocks interaction of HLA-G with inhibitoryreceptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Azide Free

Buffer: PBS, pH 7.4 without preservatives.

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles. Shelf life: One year from despatch.

Restrictions: For Research Use only