CD5 Molecule (CD5) antibody

Antigen: CD5 Molecule (CD5)

Synonyms: T1, LEU1, Ly-1, Ly-A, Ly-12, Lyt-1, MGC114334, CD5, MGC137790

Clonality: Monoclonal (CRIS1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN238398

Additional Information

Characteristics: Synonyms: Lymphocyte antigen T1/Leu-1, LEU1, T-cell surface glycoprotein CD5

Alternative name: CD5

Swiss-Prot: P06127

Immunogen: Stimulated human leukocytes.

Cross-Reactivity: Human

Isotype: IgG2a  (Matching secondary antibodies)

Clone: CRIS1

Description: CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembraneglycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset ofB-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membraneglycoprotein whose extracellular region contains three scavenger receptor cysteine-rich(SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail isdevoid of any intrinsic catalytic activity. CD5 modulates signaling through theantigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellularCa++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals dependingboth on the cell type and development stage. In thymocytes and B1a cells seems to provideinhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signalreceptor. CD5 is the phenotypic marker of a B cell subpopulation involved in theproduction of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferativedisorders (B-CLL, Hairy cell leukemia, etc. ). The CD5+ popuation is expanded in someautoimmune disorders (Rheumatoid Arthritis, etc. ). Herpes virus infections induce loss ofCD5 expression in the expanded CD8+ human T cells.

Specificity: The antibody CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chaintransmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes andB lymphocytes subset (B-1a lymphocytes). Species: Human. Others not tested.

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry: 1 μg/mlPositive Control: Peripheral Blood Lymphocytes (PBL)Jurkat human leukemia T-cell line. HPB human leukemia T-cell line. MOLT-4 human leukemia T-cell line.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only