MHC Class I H-2Dk antibody

Antigen: MHC Class I H-2Dk

Clonality: Monoclonal (H100-30-23)

Host: Mouse

Reactivity: Mouse (Murine)

Catalog no.: ABIN238410

Additional Information

Characteristics: Synonyms: H-2D(K), H2-D1, H-2 class I histocompatibility antigen, D-K alpha chain

Alternative name: MHC Class I H2 Dk

Swiss-Prot: P14426

Cross-Reactivity: Mouse (Murine)

Isotype: IgG2b  (Matching secondary antibodies)

Clone: H100-30-23

Specificity: This antibody is an MHC Class I antibody specific for cells expressing the H-2K and /or Dregion coded for by the k haplotype. It detects the H-2. m5 determinant (4). The reactionpattern of this antibody shows that it is also positive for H-2s(b,r,q) haplotypes. Species: Mouse. Others not tested. Add. Information: This reagent is not sold as sterile, but can be sterilized by filtration if necessary. Tominimize loss of volume during filtration, first dilute to the final working concentration inthe appropriate medium and then filter through a 0. 22 µ Millipore filter (or equivalent).

Application Details

Protocol: RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF H-2k BEARINGLYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in Cedarlane Cytotoxicity Medium1or equivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on density cell separation medium. After washing, adjust the cellconcentration to 1x10e7 cells per ml in Cytotoxicity Medium.

Concentration: 1x10e6 cells per mlComplement: Low toxicity Rabbit ComplementComplement 1: 15Procedure: Two stage cytotoxicity as described on page 3, Recommended Method forDetermining Percent of H-2k bearing cells in a Population.(see picture below)STRAIN DISTRIBUTION:

Purification: Ascites

Storage: Store the antibody (following reconstitution in aliquots) at -20 °C. Avoid repeated freezingand thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only