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Antibodies
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anti-CD1a Molecule (CD1A) antibody (APC)
CD1a Molecule (CD1A) antibody (APC)
| Antigen |
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| Synonyms |
R4, T6, CD1, FCB6, HTA1, CD1A, CD1.1, CD1.2, CD1A2 |
| Clonality |
Monoclonal (HI149) |
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Host
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Reactivity
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Conjugate
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FITC (15), PE (12), Biotin (11), RPE (4), APC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5,PE (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Application
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Flow Cytometry (FACS) (92), Immunohistochemistry (Frozen Sections) (IHC (fro)) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunofluorescence (IF) (22), Western Blotting (WB) (14), ELISA (7), Immunoprecipitation (IP) (6), Immunocytochemistry (ICC) (5), Immunohistochemistry (IHC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN238415 |
| Quantity |
100 Tests |
| Price |
330.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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CD1a
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Swiss-Prot
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P06126
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Immunogen
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Human thymocytes.
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Cross-Reactivity
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Human
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Isotype
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IgG1
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Clone
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HI149
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Description
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CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
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Specificity
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The antibody HI149 reacts with CD1a (T6), a 49 KDa polypeptide associated with b 2- microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.
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Application Notes
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Suitable for Flow Cytometry analysis of human blood cells using 10 m l reagent/100 m l of whole blood or 10e6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.
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Storage
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Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.
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Research Area
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Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD1a Molecule (CD1A)", type "Antibodies"
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Hosts
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Mouse (102), Rabbit (34)
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Reactivities
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Human (116), Mouse (Murine) (20), Rat (Rattus) (20), Dog (Canine) (11), Monkey (10), Pig (Porcine) (5), Cow (Bovine) (1), Horse (Equine) (1), Rabbit (1)
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Applications
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Flow Cytometry (FACS) (92), Immunohistochemistry (Frozen Sections) (IHC (fro)) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunofluorescence (IF) (22), Western Blotting (WB) (14), ELISA (7), Immunoprecipitation (IP) (6), Immunocytochemistry (ICC) (5), Immunohistochemistry (IHC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (15), PE (12), Biotin (11), RPE (4), APC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5,PE (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternatives 
