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Acrosomal Vesicle Protein 1 (ACRV1) antibody

Antigen

Acrosomal Vesicle Protein 1 (ACRV1)
Synonyms SP-10, SPACA2, D11S4365, Sp10, ACRV1, Msa63
Clonality Monoclonal (Hs-36)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunofluorescence (IF), Western Blotting (WB)
Catalog no. ABIN238422
Quantity 0.1 mg  (1.0 mg/ml)
Price 265.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
Alternative name ACRV1
Gene ID 56
Swiss-Prot P26436
Immunogen Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.
Cross-Reactivity Human
Isotype IgM  (Matching secondary antibodies)
Clone Hs-36
Description One of the most frequent causes of man infertility is defective sperm acrosome. Thisdamage can be detected using antibodies against intra-acrosomal proteins. Besidesdiagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteinscan be used for evaluation of the physiological state of sperm cells as well as for selectionof a suitable method of fertilization in the laboratories of assisted reproduction.
Specificity The antibody Hs-36 reacts with human intra-acrosomal proteins (60kDa, 25kDa)associated with the membranes of the acrosomal vesicle. Species: Human. Others not tested.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Immunocytochemistry (10 μg/ml): Membrane permeabilization (acetone) is essential. Western Blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Purified
Buffer PBS, pH 7.4 with 15 mM sodium azide as preservative
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Acrosomal Vesicle Protein 1 (ACRV1)", type "Antibodies"
Hosts Mouse (19), Rabbit (2)
Reactivities Human (13), Dog (Canine) (4), Cow (Bovine) (2), Mouse (Murine) (2), Pig (Porcine) (2)
Applications Western Blotting (WB) (20), Immunofluorescence (IF) (10), Immunocytochemistry (ICC) (7), Flow Cytometry (FACS) (6), ELISA (Detection) (2), ELISA (1)
Conjugates FITC (1)