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Antibodies
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anti-Linker For Activation of T Cells (LAT) antibody
Linker For Activation of T Cells (LAT) antibody
| Antigen |
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| Synonyms |
LAT1, pp36, MGC108666, LAT, MGC157208, lat |
| Clonality |
Monoclonal (LAT-1D1) |
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Host
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Reactivity
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Application
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 Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN238425 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-CellsFamily Member 1
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Alternative name
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LAT
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Gene ID
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27040
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Swiss-Prot
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O43561
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Immunogen
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Recombinant rat LAT.
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Cross-Reactivity
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Mouse (Murine), Rat (Rattus)
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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LAT-1D1
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Description
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LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembraneadaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Afterimmunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, orTec-family kinases, providing docking sites for downstream signaling molecules. LAT isessential for TCR-dependent T cell- and FcεRI-dependent mast cell activation, as well as formaturation of early thymocytes. It is also involved in NK cell signaling and plateletactivation.
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Specificity
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The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), atransmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells andplatelets. Negative Species: HumanSpecies: Mouse, Rat. Others not tested.
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Application Notes
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Western Blotting: 5-10 μg/mlPositive control: mouse spleen. Immunoprecipitation. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Aff - Purified
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Buffer
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PBS, pH 7.4 with 15 mM sodium azide as preservative
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Signaling, Adaptive Immunity
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Restrictions
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For Research Use only
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Alternatives for antigen "Linker For Activation of T Cells (LAT)", type "Antibodies"
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Hosts
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Mouse (24), Rabbit (12), Goat (4)
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Reactivities
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Human (36), Rat (Rattus) (8), Mouse (Murine) (7)
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Applications
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Western Blotting (WB) (29), Immunoprecipitation (IP) (13), Flow Cytometry (FACS) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), ELISA (6), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (IHC) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunofluorescence (IF) (1)
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Conjugates
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APC (2), PE (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1)
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