LIME antibody (AA 141-295) (PE)

Catalog No. ABIN238427

Product Details anti-LIME Antibody (PE)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Binding Specificity: AA 141-295

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIME antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Purification: Size-exclusion chromatography.

Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME

Clone: LIME-06

Isotype: IgG1

Application Details

Application Notes: Suitable for Flow Cytometry analysis (5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: LIME1 (LIME1 Products)

Synonyms: LIME1 antibody, DKFZp459A158 antibody, LIME antibody, LP8067 antibody, dJ583P15.4 antibody, 2810038K19Rik antibody, RGD1562795 antibody, Lck interacting transmembrane adaptor 1 antibody, LIME1 antibody, Lime1 antibody

Background: LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

Gene ID: 54923

UniProt: Q9H400