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FAS antibody (Fas (TNF Receptor Superfamily, Member 6)) (FITC)

Details for Product anti-FAS Antibody No. ABIN238437, Supplier: Log in to see
Antigen
  • A630082H08Rik
  • AI196731
  • ALPS1A
  • APO-1
  • APO1
  • APT1
  • CD95
  • FAS
  • FAS1
  • FASTM
  • lpr
  • TNFR6
  • Tnfrsf6
  • TNFRSF6
Alternatives
anti-Human FAS antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human
651
101
81
11
7
5
4
3
3
3
2
2
1
1
Host
Mouse
426
293
17
6
4
3
3
Clonality (Clone)
Monoclonal ()
Conjugate
This FAS antibody is conjugated to FITC
73
64
51
33
16
8
7
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
339
295
213
130
104
78
61
55
49
44
23
19
12
9
7
5
4
4
3
3
3
2
2
2
1
1
1
1
Supplier
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Immunogen HUT-78 human T cell lymphoma cell line
Clone LT95
Isotype IgG1
Specificity The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Characteristics Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6
Alternative Name CD95 / FAS (FAS Antibody Abstract)
Background CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6
Gene ID 355
UniProt P25445
Pathways p53 Signaling, Apoptosis, TCR Signaling
Application Notes This antibody is suitable for Flow Cytometry analysis of human blood cells using 20 μLreagent/100 μL of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Brumatti, Yon, Castro, Bueno-da-Silva, Jacysyn, Brunner, Amarante-Mendes: "Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide." in: Experimental cell research, Vol. 314, Issue 3, pp. 554-63, 2008

Guo, Zhang, Tang, Cao: "Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines." in: Blood, Vol. 106, Issue 6, pp. 2033-41, 2005

Drosopoulos, Roberts, Cermak, Sasazuki, Shirasawa, Andera, Pintzas: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, 2005

Nakamura, Kakinuma, Amada, Miyata, Taniguchi, Koda, Sato: "Pyrazole derivatives as new potent and selective 20-hydroxy-5,8,11,14-eicosatetraenoic acid synthase inhibitors." in: Bioorganic & medicinal chemistry, Vol. 12, Issue 23, pp. 6209-19, 2004

Park, Thomsen, Frevert, Pham, Skerrett, Kiener, Liles: "Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6209-16, 2003

Scaffidi, Fulda, Srinivasan, Friesen, Li, Tomaselli, Debatin, Krammer, Peter: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, 1998