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Integrin beta 2 (ITGB2) (Activation Site) antibody
| Antigen | Integrin beta 2 (ITGB2) |
| Synonyms | 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527, CD18, MFI7, LFA-1, MAC-1, CD61, GP3A, GPIIIa, INGRB3, MGC159221, MGC159223, ITGB2, integrin, ITGB3, MGC116306 |
| Epitope |
Activation Site |
| Clonality | Monoclonal (MEM-148) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
|
| Catalog no. | ABIN238447 |
| Quantity | 0.1 mg (1.0 mg/ml) |
| Price | 265.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Characteristics | Synonyms: Integrin beta-2, MFI7, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunitbeta, Complement receptor C3 subunit beta |
| Alternative name | CD18 / ITGB2 |
| Swiss-Prot | P05107 |
| Immunogen | Peripheral blood mononuclear cells. |
| Cross-Reactivity | Human |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | MEM-148 |
| Description | CD18, integrin β2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1,CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediatedby the integrin is a composite of the functions of its individual subunits. These integrinsare essential for proper leukocyte migration, mediating intercellular contacts. Absence ofCD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leadsto the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia(Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediatedcytolysis. |
| Specificity | The antibody MEM-148 recognizes the epitope exposed on free human CD18 chainsunassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin β2 subunit,β2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. Negative Species: Porcine. Species Reactivity: Tested: Human. |
Application Details
| Application Notes | Flow Cytometry: This antibody is excellent marker of activated myeloid cells. Immunoprecipitation. Western Blotting (Non-reducing conditions). Functional Application: The antibody MEM-148 induces homotypic cell aggregation andhigh-affinity LFA-1 conformation in resting leukocytes. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Concentration | 1.0 mg/ml |
| Purification | Azide Free |
| Buffer | PBS, pH 7.4 without preservatives |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles. Shelf life: One year from despatch. |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "Integrin beta 2 (ITGB2)", type "Antibodies"
| Hosts | Mouse (45), Rat (26), Rabbit (3) |
| Reactivities | Human (41), Mouse (Murine) (16), Dog (Canine) (11), Rat (Rattus) (8), Pig (Porcine) (4), Cow (Bovine) (2), Rabbit (1) |
| Applications | Flow Cytometry (FACS) (68), Immunoprecipitation (IP) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Western Blotting (WB) (20), Functional Studies (Func) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (1) |
| Conjugates | FITC (11), PE (8), Biotin (7), APC (1) |
