Integrin beta 2 (ITGB2) (Activation Site) antibody

Details for Product No. ABIN238447
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Antigen
Epitope
Activation Site
(30), (24), (16), (14), (12), (10), (7), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(258), (168), (99), (61), (53), (46), (36), (24), (1)
Host
Mouse
(180), (114), (103), (2), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(55), (40), (29), (14), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Application
Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(263), (136), (119), (63), (45), (41), (40), (40), (38), (27), (15), (6), (3), (3), (2)
Pubmed 9 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN238447
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Immunogen Peripheral blood mononuclear cells.
Clone MEM-148
Isotype IgG1
Specificity The antibody MEM-148 recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Alternative Name CD18 / ITGB2
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Alternate names: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: This antibody is excellent marker of activated myeloid cells. Immunoprecipitation. Western Blotting (Non-reducing conditions). Functional Application: The antibody MEM-148 induces homotypic cell aggregation andhigh-affinity LFA-1 conformation in resting leukocytes. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Preservative Azide free
Storage 4 °C
Storage Comment Store the antibody undiluted (in the dark) at 2-8°C. DO NOT FREEZE!!
Expiry Date 12 months
Background publications Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996 (PubMed).

Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998 (PubMed).

Drbal, Angelisová, Cerný et al.: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." in: Immunobiology, Vol. 203, Issue 4, pp. 687-98, 2001 (PubMed).

Drbal, Angelisová, Hilgert et al.: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, 2001 (PubMed).

Kess, Peters, Zamek et al.: "CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 5697-706, 2003 (PubMed).

Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005 (PubMed).

Peters, Sindrilaru, Wang et al.: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006 (PubMed).

Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007 (PubMed).

Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

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