Integrin beta 2 (ITGB2) (Activation Site) antibody (FITC)

Antigen: Integrin beta 2 (ITGB2)

Synonyms: 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527, CD18, MFI7, LFA-1, MAC-1, CD61, GP3A, GPIIIa, INGRB3, MGC159221, MGC159223, ITGB2, integrin, ITGB3, MGC116306

Epitope: Activation Site

Clonality: Monoclonal (MEM-148)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN238448

Additional Information

Characteristics: Synonyms: Integrin beta-2, MFI7, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunitbeta, Complement receptor C3 subunit beta

Alternative name: CD18 / ITGB2

Swiss-Prot: P05107

Immunogen: Peripheral blood mononuclear cells.

Cross-Reactivity: Human

Isotype: IgG1

Clone: MEM-148

Description: CD18, integrin β2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1,CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediatedby the integrin is a composite of the functions of its individual subunits. These integrinsare essential for proper leukocyte migration, mediating intercellular contacts. Absence ofCD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leadsto the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia(Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediatedcytolysis.

Specificity: The antibody MEM-148 recognizes the epitope exposed on free human CD18 chainsunassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin β2 subunit,β2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. Negative Species: Porcine. Species: Human. Others not tested.

Application Details

Application Notes: Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl ofwhole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v)high-grade BSA (protease free) as stabilizer.Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. Thereagent is free of unconjugated FITC and adjusted for direct

Storage: Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf life: One year from despatch.

Restrictions: For Research Use only