ITGAL antibody (Integrin alpha-L) (PE)

Details for Product anti-ITGAL Antibody No. ABIN238462, Supplier: Log in to see
Antigen
  • CD11A
  • LFA-1
  • LFA1A
  • (p180)
  • Cd11a
  • LFA-1A
  • Ly-15
  • Ly-21
  • CD11a
  • ITGAL
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
  • integrin alpha L
  • ITGAL
  • Itgal
Alternatives
anti-Human ITGAL antibody for Activity Inhibition
Reactivity
Human
503
225
47
23
9
7
5
4
4
2
1
1
1
Host
Mouse
508
195
80
19
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGAL antibody is conjugated to PE
97
85
60
20
19
17
17
15
15
14
13
12
11
11
11
11
10
9
9
8
5
4
4
4
3
3
2
1
1
1
1
Application
Flow Cytometry (FACS)
676
186
185
166
113
94
65
51
32
19
17
14
11
10
5
4
3
3
2
2
1
1
1
1
1
1
Options
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Immunogen Leukocytes from a pacient suffering from a LGL-type leukaemia.
Clone MEM-25
Isotype IgG1
Specificity The antibody MEM-25 reacts with CD11a (α-subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Purification Size-exclusion chromatography
Alternative Name CD11a / ITGAL (ITGAL Antibody Abstract)
Background CD11a (LFA-1α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain
Gene ID 3683
NCBI Accession NP_002200
UniProt P20701
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Activated T Cell Proliferation, Integrin Complex
Application Notes Suitable for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL ofwhole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Product cited in: Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).

Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

Background publications Fujii, Takada-Takatori, Kawashima: "Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity." in: Life sciences, Vol. 80, Issue 24-25, pp. 2320-4, 2007 (PubMed).

Giblin, Lemieux: "LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics." in: Current pharmaceutical design, Vol. 12, Issue 22, pp. 2771-95, 2006 (PubMed).

Kellersch, Kolanus: "Membrane-proximal signaling events in beta-2 integrin activation." in: Results and problems in cell differentiation, Vol. 43, pp. 245-57, 2006 (PubMed).

Sarantos, Raychaudhuri, Lum, Staunton, Simon: "Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1." in: The Journal of biological chemistry, Vol. 280, Issue 31, pp. 28290-8, 2005 (PubMed).

Mathison, Befus, Davison, Woodman: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Hajishengallis, Martin, Sojar, Sharma, Schifferle, DeNardin, Russell, Genco: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).

Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Ottonello, Epstein, Dapino, Barbera, Morone, Dallegri et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b ..." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).

Simon, Cherapanov, Nadra, Waddell, Seo, Wang, Doerschuk, Downey: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).