» Antibodies » anti-CD8a Molecule (CD8A) antibody (Biotin)

Order hotline:

	+1 404 474 4654
	+1 888 205 9894 (TF)

CD8a Molecule (CD8A) antibody (Biotin)

Antigen	CD8a Molecule (CD8A)
Synonyms	CD8, MAL, p32, Leu2, Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, MGC136643, zgc:136643, cd8, LOC100136450
Clonality	Monoclonal (MEM-87)
Host	Alternatives Mouse Mouse (310), Rat (106), Rabbit (82)
Reactivity	Alternatives Human Human (288), Mouse (Murine) (121), Rat (Rattus) (50), Cat (Feline) (21), Chicken (19), Dog (Canine) (14), Pig (Porcine) (13), Cow (Bovine) (12), Monkey (10), Sheep (Ovine) (10), Horse (Equine) (8), Rabbit (8), Guinea Pig (5), Turkey (5), Goat (4), Duck (1)
Conjugate	Alternatives Biotin FITC (84), Biotin (46), PE (41), APC (17), RPE (12), Cy5 (9), PE,Cy7 (6), PE,Cy5 (5), SPRD (5), Alexa Fluor 488 (4), HRP (4), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Flour 488 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5,PE (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), Pacific Blue (3), RBITC (3), Alexa Fluor 350 (2), Alexa Fluor 555 (2), PE,Texas Red (TR) (2), PerCP (2), APC,Cy5.5 (1), Alexa Fluor 700 (1), Cy5.5,PE (1), Cy7,PE (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), RPE,Cy5 (1), RPE,Cy5.5 (1), RPE,Cy7 (1)
Application	Alternatives Flow Cytometry (FACS) Flow Cytometry (FACS) (387), Immunohistochemistry (Frozen Sections) (IHC (fro)) (131), Immunoprecipitation (IP) (76), Immunofluorescence (IF) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), Western Blotting (WB) (43), ELISA (33), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (15), Functional Studies (Func) (7), Blocking Antibody (Inhibition) (6), Cytotoxicity Test (CyTox) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Enzyme Immunoassay (EIA) (5), Immunoelectron Microscopy (IEM) (3), ELISA (Detection) (2), To be determined by user (TBD) (1)

Catalog no.	ABIN238479
Quantity	0.1 mg (1.0 mg/ml)
Price	290.00 $ Plus shipping costs $35.00
Shipping to
Availability	Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives back to top

Characteristics	Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
Alternative name	CD8
Gene ID	925
Swiss-Prot	P01732
Immunogen	Leukocytes of a patient suffering from LGL Type Leukemia.
Cross-Reactivity	Human
Isotype	IgG1
Clone	MEM-87
Description	The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as αβ heterodimer onmajority of MHC I-restricted conventional T cells and thymocytes and as αα homodimer onsubsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8β level on T cell surface seems to be an important mechanism to controltheir effector function. Assembly of CD8 α-β but not α-α dimers is connected with formationor localization to the lipid rafts. Recruiting triggered TCR complexes to these membranemicrodomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting thefunctional diversity of the TCR signaling.
Specificity	The antibody MEM-87 recognizes CD8, a cell surface glycoprotein found on most cytotoxicT lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8is a disulfide-linked dimer and exists as a CD8α homodimer or CD8α/β heterodimer (eachmonomer approx. 32-34 kDa). Species: Human. Others not tested.

Application Details
Alternatives back to top

Application Notes	this biotinylated antibody is suitable for indirect Immunofluorescence analysis by FlowCytometry: 5-10 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration	1.0 mg/ml
Buffer	PBS, pH 7.4 containing 15 mM sodium azide as preservative.Label: Conjugated with Biotin-LC-NHS under optimum conditions.The reagent is free of unconjugated biotin.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area	Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions	For Research Use only

Alternatives
Application Details back to top

Alternatives for antigen "CD8a Molecule (CD8A)", type "Antibodies"

Hosts	Mouse (310), Rat (106), Rabbit (82)
Reactivities	Human (288), Mouse (Murine) (121), Rat (Rattus) (50), Cat (Feline) (21), Chicken (19), Dog (Canine) (14), Pig (Porcine) (13), Cow (Bovine) (12), Monkey (10), Sheep (Ovine) (10), Horse (Equine) (8), Rabbit (8), Guinea Pig (5), Turkey (5), Goat (4), Duck (1)
Applications	Flow Cytometry (FACS) (387), Immunohistochemistry (Frozen Sections) (IHC (fro)) (131), Immunoprecipitation (IP) (76), Immunofluorescence (IF) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), Western Blotting (WB) (43), ELISA (33), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (15), Functional Studies (Func) (7), Blocking Antibody (Inhibition) (6), Cytotoxicity Test (CyTox) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Enzyme Immunoassay (EIA) (5), Immunoelectron Microscopy (IEM) (3), ELISA (Detection) (2), To be determined by user (TBD) (1)
Conjugates	FITC (84), Biotin (46), PE (41), APC (17), RPE (12), Cy5 (9), PE,Cy7 (6), PE,Cy5 (5), SPRD (5), Alexa Fluor 488 (4), HRP (4), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Flour 488 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5,PE (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), Pacific Blue (3), RBITC (3), Alexa Fluor 350 (2), Alexa Fluor 555 (2), PE,Texas Red (TR) (2), PerCP (2), APC,Cy5.5 (1), Alexa Fluor 700 (1), Cy5.5,PE (1), Cy7,PE (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), RPE,Cy5 (1), RPE,Cy5.5 (1), RPE,Cy7 (1)
Epitopes	C-Term (1), N-Term (1)