HLA-E antibody

Catalog No. ABIN238484

Product Details anti-HLA-E Antibody

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-E antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: The antibody MEM-E/08 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: HLA-6.2, HLAE, MHC class I antigen E, HLA class I histocompatibility antigen, alpha chain E

Purification: Protein A-affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide. Remarks: Gene Locus: 6p21. 3

Clone: MEM-E-08

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Positive control: human cell line LCL 721.221 (HLA-E transfected). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Abstract: HLA-E Products

Synonyms: EA1.2 antibody, EA2.1 antibody, HLA-6.2 antibody, MHC antibody, QA1 antibody, major histocompatibility complex, class I, E antibody, HLA-E antibody, PATR-E antibody

Background: HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.2, HLAE, MHC class I antigen E, alpha chain E

Gene ID: 3133

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process