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CD14 Molecule (CD14) antibody

Antigen

CD14 Molecule (CD14)
Synonyms CD14
Clonality Monoclonal (Tük4)
Host
Alternatives

Mouse
Reactivity
Alternatives

Cow (Bovine)
Application
Alternatives Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Functional Studies (Func), Western Blotting (WB)
Catalog no. ABIN238505
Quantity 0.5 mg  (1.0 mg/ml)  (Variants)
Price 680.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
Alternative name CD14
Swiss-Prot P08571
Immunogen Proprietary peptide sequence.
Cross-Reactivity Cow (Bovine), Dog (Canine), Cat (Feline), Goat, Human, Monkey, Pig (Porcine), Rabbit, Sheep (Ovine)
Isotype IgG2a  (Matching secondary antibodies)
Clone Tük4
Description CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDaglycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liverderived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical forlipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivityto cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectiousdiseases and high mortality in gram negative shock. CD14 also appears to be involved inclearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
Specificity The antibody detects CD14 (low endotoxin) cell surface antigen, which is expressedstrongly by most peripheral blood monocytes and weakly on granulocytes. Species: Human. Cross recats with Cynomolgus monkey, Pig, Bovine, Sheep, Goat, Dog,Cat, Mink and Rabbit. Others not tested. Add. Information: Endotoxin level < 0. 01 Eu/µg

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes ELISA. Western blot. Flow Cytometry (1/100). Functional assay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Aff - Purified
Buffer PBS
Storage Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD14 Molecule (CD14)", type "Antibodies"
Hosts Mouse (240), Rabbit (36), Goat (8), Rat (2), Chicken (1)
Reactivities Human (265), Pig (Porcine) (33), Mouse (Murine) (30), Cow (Bovine) (27), Dog (Canine) (25), Sheep (Ovine) (24), Rabbit (23), Monkey (22), Primates (19), Rat (Rattus) (18), Goat (8), Primate (8), Cat (Feline) (7), Chicken (1), Llama (1)
Applications Flow Cytometry (FACS) (217), Western Blotting (WB) (54), ELISA (37), Immunoprecipitation (IP) (32), Functional Studies (Func) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (18), Immunohistochemistry (IHC) (16), ELISA (Detection) (5), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
Conjugates FITC (35), PE (24), Biotin (21), APC (16), RPE (8), Cy5.5 (5), Alexa Fluor 488 (3), Pacific Blue (3), Alexa Fluor 700 (2), Cy5 (2), Cy7,PE (2), PE,Cy5 (2), PE,Cy7 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), APC,Cy7 (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), CF (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Epitopes C-Term (2), N-Term (2)