TUBB antibody (Tubulin, beta)

Details for Product anti-TUBB Antibody No. ABIN238519, Supplier: Log in to see
Antigen
  • XLOT
  • m40
  • ok/sw-cl.56
  • tubb1
  • tubb5
  • 1t
  • B1t
  • BETA 56D
  • CG9277
  • DTB2
  • Dmbeta1
  • Dmel\\CG9277
  • T
  • Tub
  • Tubulin
  • beta
  • beta-Tub
  • beta-Tub56D
  • beta-tub
  • beta-tubulin56D
  • beta1
  • beta1-Tubulin
  • beta1-tub
  • beta1Tub
  • beta1t
  • beta1tub
  • beta56D
  • betaTub
  • betaTub1
  • beta[[1]] tubulin
  • beta[[1]]-tubulin
  • betatub(56D)
  • 143391_i_at
  • 3t
  • B3t
  • BETA 60D
  • CG3401
  • D.m.BETA-60D
  • DTB3
  • Dmbeta3
  • Dmel\\CG3401
  • Tub60D
  • beta-Tub60D
  • beta-Tub6D
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3TUB
  • beta3Tub
  • beta3t
  • beta60C
  • betaTub3
  • betaTub60C
  • beta[[3]] tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • betatub60D
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • 2t
  • B2t
  • BETA 85D
  • BETA2
  • CG9359
  • D.m.BETA-85D
  • DTB4
  • Dmbeta2
  • Dmel\\CG9359
  • beta(2)Tu
  • beta(2)Tub
  • beta-Tub85D
  • beta-tub85D
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta85D
  • betaTub2
  • beta[[2]]-tubulin
  • ms(3)KK[D]
  • tubB3
  • tubulin
  • Tub1
  • bmtub1
  • Tub3
  • bmtub3
  • Tub2
  • bmtub2
  • Tub4
  • bmtub4
  • LOC100101153
  • beta-5
  • tubb2a
  • tubb4
  • tub5
  • 4t
  • B4t
  • BETA 98B
  • CG4869
  • DTB1
  • Dmbeta4
  • Dmel\\CG4869
  • beta-Tub97EF
  • beta4
  • beta4t
  • beta97F
  • betaTub4
  • betaTub98BC
  • betaTub98C
  • TUBB2A
  • TUBB
  • TUBB5
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • AA408537
  • AI596182
  • B130022C14Rik
  • M(beta)5
  • TUBB2B
  • M40
  • OK/SW-cl.56
  • TUBB1
  • tubulin, beta-like
  • Tubulin, Beta
  • tubulin, beta class I
  • beta-Tubulin at 56D
  • beta-Tubulin at 60D
  • beta-Tubulin at 85D
  • beta-tubulin
  • tubulin beta
  • tubulin, beta 4A class IVa
  • tubulin, beta 2A class IIa
  • aarF domain containing kinase 5
  • putative cobalt-zinc-cadmium outer membrane resistance protein
  • beta tubulin
  • beta-5 tubulin
  • beta-Tubulin at 97EF
  • tubulin, beta
  • tubulin, beta 4A class IVA
  • tubulin, beta 5 class I
  • tubulin, beta 6 class V
  • LOC100350045
  • tbb-6
  • tubb
  • betaTub56D
  • betaTub60D
  • betaTub85D
  • tubB3
  • Tub1
  • Tub3
  • Tub2
  • Tub4
  • TVAG_525430
  • TVAG_062880
  • TVAG_456920
  • TUB3
  • TUB2
  • LOC100101153
  • tubb4a
  • TUBB2A
  • ADCK5
  • czcC
  • Tb927.1.2330
  • Tb927.1.2350
  • Tb927.1.2370
  • Tb927.1.2390
  • LOC100689088
  • TUBB5
  • LOC693049
  • LOC443015
  • betaTub97EF
  • TUBB
  • Tubb4a
  • Tubb5
  • TUBB6
Alternatives
anti-Pig (Porcine) TUBB antibody for Western Blotting
Reactivity
Human, Mouse (Murine), Plant, Pig (Porcine)
367
262
250
71
69
64
45
33
29
28
22
17
16
15
12
11
11
11
10
10
9
8
7
6
6
6
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Mouse
235
166
1
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBB antibody is un-conjugated
14
9
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
350
144
121
113
110
83
27
22
19
17
7
7
3
3
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen beta-tubulin from porcine brain.
Clone TU-13
Isotype IgM
Specificity The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.
Characteristics Synonyms: Tubulin beta chain, Tubulin beta-5 chain
Purification Precipitation Methods and Size-Exclusion Chromatography.
Alternative Name TUBB / TUBB5 (TUBB Antibody Abstract)
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.Synonyms: Tubulin beta chain, Tubulin beta-5 chain
Gene ID 203068
UniProt P07437
Pathways Microtubule Dynamics, M Phase
Application Notes Western Blotting (Reducing Conditions): 1 μg/mL, Sonication of MCF7 cells isrecommended. Positive Control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 with 15 mM Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch
Expiry Date 12 months
Background publications Linhartová, Dráberová, Viklický, Dráber: "Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells." in: FEBS letters, Vol. 320, Issue 1, pp. 79-82, 1993 (PubMed).

Dráber, Vater, Böhm, Kuklova, Unger: "Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies." in: FEBS letters, Vol. 262, Issue 2, pp. 209-11, 1990 (PubMed).

Dráber, Lagunowich, Dráberová, Viklický, Damjanov: "Heterogeneity of tubulin epitopes in mouse fetal tissues." in: Histochemistry, Vol. 89, Issue 5, pp. 485-92, 1988 (PubMed).

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