Tubulin gamma 1 (TUBG1) antibody (DY647)

Antigen: Tubulin gamma 1 (TUBG1)

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Clonality: Monoclonal (TU-30)

Host: Mouse

Reactivity: Chicken

Conjugate: DY647

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN238520

Additional Information

Characteristics: Synonyms: Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1

Alternative name: TUBG1 / Tubulin gamma 1

Swiss-Prot: P23258

Immunogen: C-terminal peptide (aa 434-449) of gamma-tubulin counjugated to KLHAA Sequence: EYHAATRPDYISWGTQ

Cross-Reactivity: Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Protozoa

Isotype: IgG1

Clone: TU-30

Description: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member oftubulin family (less that 0. 01% of tubulin dimer). The gamma-tubulin ring structures,however, serve to provide structural primer for initiation of microtubular nucleation andgrowth, thereby being crutial for microtubule-based cellular processes, above all formitotic spindle formation. In animal cells, a center of microtubule organization is thecentrosome composed of a pair of cylindrical centrioles surrounded by fibrouspericentriolar material containing gamma-tubulin. Formation of the mitotic spindle ispreceded by duplication of centrosome during S phase. Before mitosis, both centrosomesincrease their microtubule nucleation capacity and form two microtuble asters that arepushed apart from each other by the forces of motor proteins associated at themicrotubule surface.

Specificity: The antibody TU-30 recognizes C-terminal peptide sequence (aa 434-449) ofgamma-Tubulin. Species: Human, Porcine, Mouse, Rat, Chicken, Protozoa and Plants. Others not tested.

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Application Details

Application Notes: Immunocytochemistry on Fixed and Permeabilized Cells (1/100). Immunohistochemistry on Paraffin Sections using confocal microscopy. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: Phosphate Buffered Saline (PBS), pH~7.4 with 15 mM Sodium Azide aspreservative.Label: Conjugated with Dyomics 647 (DY647) under optimum conditions

Storage: Store the antibody undiluted at 2-8°CDO NOT FREEEZE! Avoid prolonged exposure to light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only