HLA Class I ABC antibody

Antigen: HLA Class I ABC

Clonality: Monoclonal (W6-32)

Host: Mouse

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN238527

Additional Information

Characteristics: Synonyms: HLA-ABC, HLA class I histocompatibility antigen, MHC class I antigen

Immunogen: Membrane of human tonsil cells. Remarks: Gene Locus: 6p21. 3

Cross-Reactivity: Cow (Bovine), Cat (Feline), Human

Isotype: IgG2a  (Matching secondary antibodies)

Clone: W6-32

Description: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta-2microglobulin. They carry foreign determinants important for immune recognition bycytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class Iantigens are represented by HLA-A, HLA-B and HLA-C molecules. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.

Specificity: The antibody W6/32 recognizes virtually all nucleated human cells. It W6/32 is a valuablereagent for analysing variations in HLA class I expression in different disease states e. g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromusculardisorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing andcrossmatching. Species: Human, Non-Human Primates, Bovine, Feline (Cat). Does not work in Rabbit. Others not tested.

Application Details

Application Notes: ELISA. Flow Cytometry. Immunoprecipitation. Functional Application. Western blot (non Reducing Conditions). Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS), preserved by filter sterilization, ~pH 7.4without preservatives.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only