Ligand of Numb-Protein X 1 (LNX1) antibody

Antigen: Ligand of Numb-Protein X 1 (LNX1)

Synonyms: LNX, MPDZ, PDZRN2, Lnx, LNX1, lnx, fj78f06, MGC152906, wu:fj78f06, zgc:152906, si:dkey-12h2.1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238544

Additional Information

Alternative name: LNX1

Gene ID: 84708

Immunogen: C-PDAYRPRDDSFH

Format: Affinity-purified

Description: All names and symbols: LNX1, ligand of numb-protein X 1 , LNX, MPDZ, PDZRN2 , multi-PDZ-domain-containing protein. Official Symbol: LNX1. RefSeq number(s): NP_116011.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave next to 70kDa bands at approx 75kDa and 27kDa in Human Kidney and Lung lysates after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 69.7kDa according to NP_116011.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Chen, Xu, Zhao et al.: "Characterization of human LNX, a novel ligand of Numb protein X that is downregulated in human gliomas." in: The international journal of biochemistry & cell biology, Vol. 37, Issue 11, pp. 2273-83, 2005 (PubMed).