Chromodomain Helicase DNA Binding Protein 7 (CHD7) (Internal Region) antibody

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Antigen
Synonyms
CHD7, fd19h06, si:ch211-197o6.2, wu:cegs2051, wu:fb37f10, wu:fb39h04, wu:fd19h06, HH5, IS3, KAL5, A730019I05Rik, Cycn, Cyn, Dz, Edy, Flo, GENA 47, GENA 60, Gena 52, Lda, Mt, Obt, Todo, WBE1, Whi, meti ... show more
CHD7, fd19h06, si:ch211-197o6.2, wu:cegs2051, wu:fb37f10, wu:fb39h04, wu:fd19h06, HH5, IS3, KAL5, A730019I05Rik, Cycn, Cyn, Dz, Edy, Flo, GENA 47, GENA 60, Gena 52, Lda, Mt, Obt, Todo, WBE1, Whi, metis show less
Epitope
Internal Region
(13), (5), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(25), (7), (4), (1), (1), (1), (1), (1)
Host
Goat
(17), (9), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(19), (11), (4), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-KDEIDEFANSPSE, from the internal region of the protein sequence according to NP_060250.2.
Blocking Peptide Blocking peptide for this product available: Chromodomain Helicase DNA Binding Protein 7 (CHD7) peptide (ABIN369244)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CHD7 (CHD7 Antibody Abstract)
Background Synonyms: chromodomain helicase DNA binding protein 7, FLJ20357, FLJ20361, IS3, KIAA1416, Scoliosis, idiopathic 3
Gene ID 55636, 320790, 312974
NCBI Accession NP_060250
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 250 kDa and 48 kDa in Rat Spinal Cord lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 336 kDa according to Human NP_060250.2 and 175 kDa according to predicted Rat NP_001101376.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Gao, Gordon, Zhang et al.: "CHD7 gene polymorphisms are associated with susceptibility to idiopathic scoliosis." in: American journal of human genetics, Vol. 80, Issue 5, pp. 957-65, 2007 (PubMed).

Catalog No. ABIN238550
$318.20
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