Group IB Phospholipase A2 (PLA2G1B) antibody

Antigen: Group IB Phospholipase A2 (PLA2G1B)

Synonyms: PLA2, PLA2A, PPLA2, MGC119834, MGC119835, Pla2a, MGC6679, sPLA2IB

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238555

Additional Information

Alternative name: PLA2G1B

Gene ID: 5319, 18778 (mouse), 29526 (rat)

Immunogen: NKAHKNLDTKKYCQS

Format: Affinity-purified

Description: All names and symbols: PLA2G1B, phospholipase A2, group IB (pancreas), MGC119834, MGC119835, PLA2, PLA2A, PPLA2, phospholipase A2, group IB,. Official Symbol: PLA2G1B . RefSeq number(s): NP_000919.1

Specificity: Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism, Enzymes

Restrictions: For Research Use only

Images

ABIN238555 (0.01µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing PLA2G1B (RC216089) and probed with ABIN238555 (mock transfection in first lane), tested by Origene.

Publications

Publications: Kolko, Christoffersen, Varoqui et al.: "Expression and induction of secretory phospholipase A2 group IB in brain." in: Cellular and molecular neurobiology, Vol. 25, Issue 7, pp. 1107-22, 2006 (PubMed).