Corticotropin Releasing Hormone Receptor 1 (CRHR1) (AA 107-117) antibody

Antigen: Corticotropin Releasing Hormone Receptor 1 (CRHR1)

Synonyms: CRF1, CRHR, CRF-R, CRFR1, CRHR1f, CRH-R1h, CRFR2, crhr2, CRF2, crhr2-A, Crhr, MGC124237, MGC124240, CRHR1, crhr1-A

Epitope: AA 107-117

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN238557

Additional Information

Alternative name: CRHR1 / CRF-R (aa 107 - 117)

Gene ID: 1394, 12921 (mouse), 58959 (rat)

Immunogen: C-NEEKKSKVHYH

Format: Affinity-purified

Description: All names and symbols: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor. Official Symbol: CRHR1 . RefSeq number(s): NP_004373.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60-70kDa band observed in human brain (cerebellum) and human colon lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar,59(3):485-92, PMID: 11179443) . Recommended concentration: 0.1-0.3µg/ml. This product was successfully used in immunofluorescence on mouse brain at 2ug/ml when incubated at 4C for 40h.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238557 (0.1µg/ml) staining of human colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Confocal image (3µm) of ABIN238557 (0.3µg/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.

Publications

Publications: Rissman, Lee, Vale et al.: "Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 24, pp. 6552-62, 2007 (PubMed).