Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 1 (KCNB1) antibody

Antigen: Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 1 (KCNB1)

Synonyms: DRK1, KV2.1, h-DRK1, Kcnb1, KCNB1, Shab

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238567

Additional Information

Alternative name: KCNB1 / DRK1

Gene ID: 3745, 16500 (mouse), 25736 (rat)

Immunogen: C-HQYIDADTDDEGQ

Format: Affinity-purified

Description: All names and symbols: KCNB1, potassium voltage-gated channel, Shab-related subfamily, member 1, DRK1, KV2.1, h-DRK1, delayed rectifier potassium channel Kv2.1, h-DRK1 K channel, potassium channel protein DRK1, potassium voltage-gated channel subfamily B member 1, voltage-gated potassium channel. Official Symbol: KCNB1. RefSeq number(s): NP_004966.1

Specificity: Human, Mouse, Dog, Cow, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave a 100kDa band and additional stronger bands at approx 50kDa and 38kDa in Human Brain (Cerebellum) and Heart lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 95.9kDa according to NP_004966.1. Both detected extra bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Park, Mohapatra, Misonou et al.: "Graded regulation of the Kv2.1 potassium channel by variable phosphorylation." in: Science (New York, N.Y.), Vol. 313, Issue 5789, pp. 976-9, 2006 (PubMed).