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Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 1 (KCNB1) (Internal Region) antibody

Details for Product No. ABIN238567, Supplier: Log in to see
Antigen
  • DRK1
  • DRK1PC
  • h-DRK1
  • Kcnb1
  • KCNB1
  • Kcr1-1
  • KV2.1
  • Kv2.1
  • Shab
Epitope
Internal Region
15
12
12
6
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Dog (Canine), Cow (Bovine), Pig (Porcine)
73
50
50
6
3
3
3
2
1
1
1
Host
Goat
55
14
12
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
50
36
20
11
11
9
7
6
5
5
1
Supplier
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Immunogen Peptide with sequence C-HQYIDADTDDEGQ, from the internal region of the protein sequence according to NP_004966.1.
Blocking Peptide Blocking peptide for this product available: ABIN369200
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KCNB1 / DRK1 (KCNB1 Antibody Abstract)
Background Synonyms: KCNB1, potassium voltage-gated channel, Shab-related subfamily, member 1, DRK1, KV2.1, h-DRK1, delayed rectifier potassium channel Kv2.1, h-DRK1 K channel, potassium channel protein DRK1, potassium voltage-gated channel subfamily B member 1, voltage-gated potassium channel
Gene ID 3745, 16500, 25736
NCBI Accession NP_004966
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments gave a 100 kDa band and additional stronger bands at approx 50 kDa and 38 kDa in Human Brain (Cerebellum) and Heart lysates after 0.5 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 95.9 kDa according to NP_004966.1. Both detected extra bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Park, Mohapatra, Misonou, Trimmer: "Graded regulation of the Kv2.1 potassium channel by variable phosphorylation." in: Science (New York, N.Y.), Vol. 313, Issue 5789, pp. 976-9, 2006

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