MEPCE antibody (Internal Region)

Catalog No. ABIN238571

Product Details anti-MEPCE Antibody

Target: MEPCE (Methylphosphate Capping Enzyme (MEPCE))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MEPCE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MEPCE

Sequence: KQQRKFQYGN YCK

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: KQQRKFQYGNYCK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx. 75 kDa band observed in Rat Testis lysates (calculated MW of 74.4 kDa according to Human NP_062552.2 and 75.3 kDa according to Rat XP_222048.4). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Preliminary testing wa

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MEPCE

Target: MEPCE (Methylphosphate Capping Enzyme (MEPCE))

Alternative Name: MEPCE (MEPCE Products)

Background: MEPCE, methylphosphate capping enzyme, BCDIN3, FLJ20257, bin3, bicoid-interacting 3, bin3, bicoid-interacting 3, homolog

Gene ID: 56257

NCBI Accession: NP_062552

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection