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HYPB / SETD2 (Internal Region) antibody

Antigen

HYPB / SETD2

Epitope

Internal Region

Clonality Polyclonal
Host

Goat

Reactivity

Mouse (Murine)

Application
ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN238587
Quantity 100µg  (100µg/200µl)
Price 369.20 $   Plus shipping costs $35.00
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Additional Information

Gene ID 29072, 235626 (mouse)
Swiss-Prot NP_054878.3
Immunogen Peptide with sequence C-ERDPDKQTQNKE, from the internal region of the protein sequence according to NP_054878.3.
Cross-Reactivity Human, Mouse (Murine)
Format Liquid
Grade Serum-free
Description Official Symbol: SETD2
Specificity Human, Mouse

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 290kDa band observed in lysates of mouse fybroblasts with a genetic construct encoding siRNA. The observed band is knocked down by two different siRNA molecules. Calculated MW of 231kDa according to NP_054878.3). Data obtained from anonymous customer. The Ponceau red demonstrates proper protein loading. Recommended concentration: 0.5-2µg/ml.
Concentration 100µg/200µl
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Sun, Wei, Wu et al.: "Identification and characterization of a novel human histone H3 lysine 36-specific methyltransferase." in: The Journal of biological chemistry, Vol. 280, Issue 42, pp. 35261-71, 2005 (PubMed).