Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP) (AA 662-672) antibody

Antigen: Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP)

Synonyms: COL4A3BP, col4a3bp, zgc:92147, wu:fc37f11, CERT, GPBP, AU016711, 2810404O15Rik, 9230101K08Rik, CERTL, STARD11, FLJ20597, cetp, MGC80009

Epitope: AA 662-672

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238598

Additional Information

Alternative name: COL4A3BP (aa662-672)

Gene ID: 10087, 68018 (mouse)

Immunogen: DHDSAPLNNRC

Format: Affinity-purified

Description: All names and symbols: COL4A3BP, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein , CERT, CERTL, FLJ20597, GPBP , STARD11, Goodpasture antigen-binding protein, START domain containing 11, alpha 3 type IV collagen binding protein, ceramide transporter,. Official Symbol: COL4A3BP. RefSeq number(s): NP_005704.1, NP_112729.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1) Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in mouse heart lysates (calculated MW of 71.1kDa according to mouse NP_075909.1 and 60.0kDa according to human NP_112729.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238598 (0.5µg/ml) staining of mouse heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Swanton, Marani, Pardo et al.: "Regulators of mitotic arrest and ceramide metabolism are determinants of sensitivity to paclitaxel and other chemotherapeutic drugs." in: Cancer cell, Vol. 11, Issue 6, pp. 498-512, 2007 (PubMed).