Diaphanous Homolog 3 (Drosophila) (DIAPH3) (C-Term), (Internal Region) antibody

Details for Product No. ABIN238601
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Antigen
Synonyms Diap3, DIAP3, DIAPH3, 4930417P13Rik, Dia2, Diaph3, Drf3, mDia2, mKIAA4117, p134MDia2, AN, AUNA1, DIA2, DRF3, NSDAN, diap3
Epitope
C-Term, Internal Region
(9), (8), (4), (2), (2), (1), (1)
Reactivity
Human
(25), (3), (3)
Host
Goat
(19), (4), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(22), (13), (4), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN238601
261.61 $
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Immunogen Peptide with sequence C-RTPMPKDVRQS, from the internal region (near the C terminus) of the protein sequence according to NP_001035982.1, NP_112194.2.
Specificity This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DIAPH3
Background Synonyms: DIAPH3, diaphanous homolog 3 (Drosophila), RP11-26P21.1, DKFZP434C0931, DKFZp686A13178, DRF3, FLJ34705, diap3, diaphanous homolog 3, diaphanous-related formin 3
Gene ID 81624
NCBI Accession NP_112194, NP_001035982
Research Area Signaling, Cytoskeleton, Microfilaments
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 50 kDa band in human bone marrow, liver and lymph node lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137 kDa according to NP_001035982.1 and 98.1 kDa according to NP_112194.2. The ~50 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Yang, Czech, Gerboth et al.: "Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells." in: PLoS biology, Vol. 5, Issue 11, pp. e317, 2007 (PubMed).

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