Diaphanous Homolog 3 (Drosophila) (DIAPH3) (C-Term), (Internal Region) antibody

Details for Product No. ABIN238601
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Synonyms AN, AUNA1, DIA2, DRF3, NSDAN, diap3, mDia2, 4930417P13Rik, Dia2, Diaph3, Drf3, mKIAA4117, p134MDia2, Diap3, DIAP3, DIAPH3
C-Term, Internal Region
(4), (3), (2), (2), (2), (1)
(19), (3), (3)
(13), (4), (3)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(16), (7), (4), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN238601
Quantity 100 µg
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Immunogen Peptide with sequence C-RTPMPKDVRQS, from the internal region (near the C terminus) of the protein sequence according to NP_001035982.1, NP_112194.2.
Specificity This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DIAPH3
Background Synonyms: DIAPH3, diaphanous homolog 3 (Drosophila), RP11-26P21.1, DKFZP434C0931, DKFZp686A13178, DRF3, FLJ34705, diap3, diaphanous homolog 3, diaphanous-related formin 3
Gene ID 81624
NCBI Accession NP_001035982.1, NP_112194.2
Research Area Signaling, Cytoskeleton, Microfilaments
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 50 kDa band in human bone marrow, liver and lymph node lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137 kDa according to NP_001035982.1 and 98.1 kDa according to NP_112194.2. The ~50 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Yang, Czech, Gerboth et al.: "Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells." in: PLoS biology, Vol. 5, Issue 11, pp. e317, 2007 (PubMed).

Hosts (13), (4), (3)
Reactivities (19), (3), (3)
Applications (16), (7), (4), (2), (1), (1), (1)
Epitopes (4), (3), (2), (2), (2), (1)
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