Diaphanous Homolog 3 (Drosophila) (DIAPH3) antibody

Antigen: Diaphanous Homolog 3 (Drosophila) (DIAPH3)

Synonyms: Diap3, DRF3, diap3, mDia2, FLJ34705, DKFZp434C0931, DKFZp686A13178, DIAP3, DIAPH3, DKFZp468P0520, Dia2, Drf3, KIAA4117, mKIAA4117, p134MDia2, 4930417P13Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN238601

Additional Information

Alternative name: DIAPH3

Gene ID: 81624

Immunogen: C-RTPMPKDVRQS

Description: All names and symbols: DIAPH3, diaphanous homolog 3 (Drosophila), RP11-26P21.1, DKFZP434C0931, DKFZp686A13178, DRF3, FLJ34705, diap3, diaphanous homolog 3, diaphanous-related formin 3. Official Symbol: DIAPH3. RefSeq number(s): NP_001035982.1, NP_112194.2

Specificity: This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 50kDa band in human bone marrow, liver and lymph node lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to NP_001035982.1 and 98.1kDa according to NP_112194.2. The ~50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Publications

Yang, Czech, Gerboth et al.: "Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells." in: PLoS biology, Vol. 5, Issue 11, pp. e317, 2007 (PubMed).