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SRY (Sex Determining Region Y)-Box 17 (SOX17) (Internal Region) antibody

Details for Product No. ABIN238613, Supplier: Login to see
Antigen
  • sox17
  • Xsox17
  • sox17beta
  • sox17-beta
  • tSox17beta
  • Xsox17-beta
  • SOX17
  • Sox17alpha
  • Xsox17-alpha
  • Xsox17alpha
  • sox17-alpha
  • tSox17alpha
  • xSox17alpha1
  • xSox17alpha2
  • xsox17a
  • sox7/17
  • VUR3
  • Sox
Epitope
Internal Region
25
17
8
4
4
2
2
1
1
1
Reactivity
Human, Rat (Rattus)
69
16
7
6
3
3
3
1
1
1
Host
Goat
31
27
19
Clonality
Polyclonal
Conjugate
Un-conjugated
3
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
45
32
17
9
8
7
6
6
3
1
1
1
1
Supplier
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Immunogen Peptide with sequence EALPCRDGTDP, from the internal region of the protein sequence according to NP_071899.1.
Blocking Peptide Blocking peptide for this product available: ABIN370105
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SOX17 (SOX17 Antibody Abstract)
Background Synonyms: SRY (sex determining region Y)-box 17, FLJ22252, SRY-box 17, SRY-related HMG-box transcription factor SOX17
Gene ID 64321, 312936
NCBI Accession NP_071899
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 20 kDa band in Rat Brain and Rat Spinal Cord lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.6 kDa according to Mouse NP_035571.1 and of 44.1 kDa according to Human NP_071899.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Kim, Saunders, Morrison: "Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells." in: Cell, Vol. 130, Issue 3, pp. 470-83, 2007 (PubMed).