SRY (Sex Determining Region Y)-Box 17 (SOX17) (Internal Region) antibody

Details for Product No. ABIN238613
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Antigen
Synonyms sox17, Xsox17, sox17beta, sox17-beta, tSox17beta, Xsox17-beta, SOX17, Sox17alpha, Xsox17-alpha, Xsox17alpha, sox17-alpha, tSox17alpha, xSox17alpha1, xSox17alpha2, xsox17a, sox7/17, VUR3, Sox
Epitope
Internal Region
(3), (3), (2), (2), (2), (1)
Reactivity
Human, Rat (Rattus)
(45), (17), (14), (12), (12), (12), (5), (4), (2), (1), (1), (1)
Host
Goat
(24), (18), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(25), (11), (10), (10), (8), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN238613
261.61 $
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Immunogen Peptide with sequence EALPCRDGTDP, from the internal region of the protein sequence according to NP_071899.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SOX17
Background Synonyms: SRY (sex determining region Y)-box 17, FLJ22252, SRY-box 17, SRY-related HMG-box transcription factor SOX17
Gene ID 64321, 312936
NCBI Accession NP_071899
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 20 kDa band in Rat Brain and Rat Spinal Cord lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.6 kDa according to Mouse NP_035571.1 and of 44.1 kDa according to Human NP_071899.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Kim, Saunders, Morrison: "Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells." in: Cell, Vol. 130, Issue 3, pp. 470-83, 2007 (PubMed).

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