SRY (Sex Determining Region Y)-Box 17 (SOX17) antibody

Antigen: SRY (Sex Determining Region Y)-Box 17 (SOX17)

Synonyms: MGC91776, FLJ22252, sox17, Xsox17, sox17beta, sox17-beta, tSox17beta, Xsox17-beta, SOX17, xsox17a, Sox17alpha, sox17-alpha, tSox17alpha, Xsox17-alpha, xSox17alpha1, xSox17alpha2, sox17a, xsox17, sox17a1, MGC131036, xsox17-alpha, sox17b, sox17b.1, Sox17beta, XSox17beta, xsox17-beta, sox17a2, MGC68799, Xsox17alpha, sox17b.3, sox7/17

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238613

Additional Information

Alternative name: SOX17

Gene ID: 64321, 312936 (rat)

Immunogen: EALPCRDGTDP

Format: Affinity-purified

Description: All names and symbols: SRY (sex determining region Y)-box 17, FLJ22252, SRY-box 17, SRY-related HMG-box transcription factor SOX17. Official Symbol: SOX17. RefSeq number(s): NP_071899.1

Specificity: Human, Rat

Synonyms: MGC91776, FLJ22252, sox17, Xsox17, sox17beta, sox17-beta, tSox17beta, Xsox17-beta, SOX17, xsox17a, Sox17alpha, sox17-alpha, tSox17alpha, Xsox17-alpha, xSox17alpha1, xSox17alpha2, sox17a, xsox17, sox17a1, MGC131036, xsox17-alpha, sox17b, sox17b.1, Sox17beta, XSox17beta, xsox17-beta, sox17a2, MGC68799, Xsox17alpha, sox17b.3, sox7/17

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 20kDa band in Rat Brain and Rat Spinal Cord lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.6kDa according to Mouse NP_035571.1 and of 44.1kDa according to Human NP_071899.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Embryogenesis, Embryonic stem cells, Transcription Factors

Restrictions: For Research Use only

Publications

Publications: Kim, Saunders, Morrison: "Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells." in: Cell, Vol. 130, Issue 3, pp. 470-83, 2007 (PubMed).