Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM) antibody

Antigen: Protein Tyrosine Phosphatase, Receptor Type, M (PTPRM)

Synonyms: RPTPM, RPTPU, PTPRL1, hR-PTPu, R-PTP-MU, MGC166994, RPTPmu, KIAA4044, MGC90724, mKIAA4044, cb984, zgc:110218, PTPRM, ptprm, DKFZp459F233

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238629

Additional Information

Alternative name: PTPRM

Gene ID: 5797

Immunogen: C-KLIRQVDKWQEEYN

Format: Affinity-purified

Description: All names and symbols: PTPRM, protein tyrosine phosphatase, receptor type, M, MGC166994, PTPRL1, R-PTP-MU, RPTPM, RPTPU, hR-PTPu, protein tyrosine phosphatase mu, protein tyrosine phosphatase, receptor type, mu polypeptide. Official Symbol: PTPRM. RefSeq number(s): NP_001098714.1, NP_002836.3

Specificity: This antibody is expected to recognise both reported isoforms (NP_001098714.1 and NP_002836.3). Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave bands at approx 110kDa and 16kDa in Rat Retina lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 165kDa according to Human NP_001098714.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Kinases/Phosphatases

Restrictions: For Research Use only

Publications

Publications: Aricescu, Hon, Siebold et al.: "Molecular analysis of receptor protein tyrosine phosphatase mu-mediated cell adhesion." in: The EMBO journal, Vol. 25, Issue 4, pp. 701-12, 2006 (PubMed).