3-hydroxybutyrate Dehydrogenase, Type 2 (BDH2) (AA 60-71) antibody

Antigen: 3-hydroxybutyrate Dehydrogenase, Type 2 (BDH2)

Synonyms: DHRS6, EFA6R, SDR15C1, UCPA-OR, UNQ6308, FLJ13261, PRO20933, Dhrs6, 1810026B04Rik, BDH2, MGC127950, zgc:110323, bdh2, MGC131325

Epitope: AA 60-71

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238652

Additional Information

Alternative name: BDH2 / DHRS6 (aa 60 to 71)

Gene ID: 56898

Immunogen: C-TKKKQIDQFANE

Format: Affinity-purified

Description: All names and symbols: BDH2, DHRS6, 3-hydroxybutyrate dehydrogenase, type 2, EFA6R, FLJ13261, PRO20933, UCPA-OR, UNQ6308, dehydrogenase/reductase (SDR family) member 6, oxidoreductase UCPA. Official Symbol: BDH2 . RefSeq number(s): NP_064524.3

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 26kDa band observed in Human Brain (Cerebellum), Kidney and Liver lysates (calculated MW of 26.7kDa according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238652 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing BDH2 (RC210586) and probed with ABIN238652 (mock transfection in first lane), tested by Origene.

Publications

Publications: Guo, Lukacik, Papagrigoriou et al.: "Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase." in: The Journal of biological chemistry, Vol. 281, Issue 15, pp. 10291-7, 2006 (PubMed).

Buhrke, Lengler, Lampen: "Analysis of proteomic changes induced upon cellular differentiation of the human intestinal cell line Caco-2." in: Development, growth & differentiation, Vol. 53, Issue 3, pp. 411-26, 2011 (PubMed).