Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6) (Internal Region) antibody

Details for Product No. ABIN238654
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Antigen
Synonyms mKIAA0597, 3830408G03, F830029L24Rik, DOA10, MARCH-VI, RNF176, TEB4, RGD1310927, RGD1565757, im:7161807, wu:fa98a02, si:dkey-40c17.2, Mar-06
Epitope
Internal Region
(3), (3), (3), (3)
Reactivity
Human
(29), (20), (17), (17), (17), (17), (17)
Host
Goat
(17), (9), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(15), (14), (12), (10), (5)
Pubmed 1 reference available
Catalog no. ABIN238654
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-QGRRVIFQKVKE, from the internal region of the protein sequence according to NP_005876.2.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative Name TEB4 / MARCH-VI
Background Synonyms: MARCH-VI, TEB4, MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, RNF176, membrane-associated RING-CH protein VI
Gene ID 10299, 223455
NCBI Accession NP_005876.2
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 100 kDa band observed in Human Lung lysates (calculated MW of 103 kDa according to NP_005876.2). Recommended concentration: 0.2-0.5 µg/mL. An additional band of unknown identity was also consistently observed at 18 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6) (Internal Region) antibody ABIN238654 (0.2 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Kreft, Wang, Hochstrasser: "Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 4646-53, 2006 (PubMed).

Hosts (17), (9), (5)
Reactivities (29), (20), (17), (17), (17), (17), (17)
Applications (15), (14), (12), (10), (5)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (3), (3), (3)
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