Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6) (Internal Region) antibody

Details for Product No. ABIN238654
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Antigen
Synonyms RGD1310927, RGD1565757, im:7161807, wu:fa98a02, si:dkey-40c17.2, Mar-06, 3830408G03, F830029L24Rik, mKIAA0597, DOA10, MARCH-VI, RNF176, TEB4
Epitope
Internal Region
(5), (3), (3), (3), (1), (1)
Reactivity
Human
(28), (16), (13), (13), (13), (13), (13)
Host
Goat
(14), (9), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(14), (13), (11), (10), (5), (1)
Pubmed 1 reference available
Quantity 100 μg
Options
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Catalog No. ABIN238654
374.27 $
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Immunogen Peptide with sequence C-QGRRVIFQKVKE, from the internal region of the protein sequence according to NP_005876.2.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TEB4 / MARCH-VI
Background Synonyms: MARCH-VI, TEB4, MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, RNF176, membrane-associated RING-CH protein VI
Gene ID 10299, 223455
NCBI Accession NP_005876
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 100 kDa band observed in Human Lung lysates (calculated MW of 103 kDa according to NP_005876.2). Recommended concentration: 0.2-0.5 µg/mL. An additional band of unknown identity was also consistently observed at 18 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6) (Internal Region) antibody ABIN238654 (0.2 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Kreft, Wang, Hochstrasser: "Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 4646-53, 2006 (PubMed).

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